Rapid test for detection of E. coli in milk
Technology Details:
“Two-stage test” has been developed for detection of E. coli based on the principle of targeting enzyme substrate reaction for specific marker enzyme (s) to release free chromogen in stage-1 which can be visually detected by a color change after 12±1.0h of incubation in E. coli. In stage-2 using specific enzyme substrate mixtures, confirmation of E. coli can be achieved within 3.00±0.15h. The developed test can be used in dairy industry for routine detection of E. coli in milk and milk products for regulatory compliance. The appearance of blue color in Stage-1 and stage -2 indicates the resumptive/confirmatory presence of E. coli. The novel features of the technology are as follows: Novel medium is selective in terms of inhibition of contaminants like Salmonella, Shigella, Yersinia, Proteus, Serratia, Citrobacter, Enterobacter, L. monocytogenes, B. cereus, S. aureus, L. casei other than E. coli spp. The developed enzyme assay for E. coli can confirm <1.0 log CFU/ml within 12.0±1.0h for presumptive detection and 3.0±0.15h for its confirmatory detection as against 4 days protocol followed in the conventional method (IS: 5887 Part-1: 1976). Two stage assay can be used for regulatory compliance of food samples including raw, pasteurized, dried milk and other food products as specified in FSS Act. 2015.Lab Validation of developed kit with IS: 5887 Part-1:1976 using raw, pasteurized and dried milk.